Coding

Part:BBa_K1194005:Design

Designed by: Aman Kumar   Group: iGEM13_IIT_Madras   (2013-09-26)


pLuxR --> I3A gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


Design Notes

The gene sequences were codon optimized for the optimal expression in bacterial system on induction with AHL (N-acyl homoserine lactone).


Source

The gene sequence for tryptophan transaminase is derived from Arabidopsis Thaliana. The gene sequence for indole pyruvate decarboxylase is taken from Azospirillum brasilense. The sequence for pLuxR is taken from Registry.

References